Temperature dependent protein unfolding
نویسنده
چکیده
Protein structure prediction is a central problem in current biophysics. Understanding the energy landscape roughness would shed light on the physics behind protein folding. The theory for extracting energy landscape roughness from temperature dependent unfolding measurements is reviewed. Improvements facilitating temperature dependent single molecule protein unfolding experiments are described. We discuss the automation of AFM stepper motor control, and present an experiment server and an automatic force curve sorting algorithm.
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